Thoracic Research and Practice
Clinical Study

Angiotensin Converting Enzyme 1 Gene Insertion/Deletion Polymorphism in Patients with Sarcoidosis

1.

University of İstanbul, Institute for Experimental Medicine, Departments of Genetic, Istanbul, Turkey

2.

University of İstanbul, Faculty of Medicine, Deparment of Respiratory Medicine, Istanbul, Turkey

3.

University of Istanbul, Faculty of Medicine, Department of Public Health, Istanbul, Turkey

Thorac Res Pract 2001; 2: Turkish Respiratory Journal 35-38
Read: 467 Downloads: 303 Published: 07 October 2021

Objective: Serum angiotensin converting enzyme (SAGE) increases in various diseases including sarcoidosis and tuberculo­sis. Genetic control of SAGE levels has been suggested by the identification of ACE gene insertion/deletion (I/D) polymor­phism. Furthermore ACE gene I/D polymorphism suggested as a marker of risk in the sarcoidosis. In the present study, we investi­gated the probable association between the ACE gene I/D poly­morphism and development of sarcoidosis.

Methods: We analyzed the I/D polymorphism of the ACE gene in 48 patients with sarcoidosis and 51 patients with tuberculosis. DNA was extracted from the white cells and the I/D polymor­phism of the ACE gene was detected by polimerase chain reaction (PCR). In homozygous DD samples, a second PCR reaction with primers that recognize an insertion spesific sequence was per­formed. The frequencies of the ACE gene’s alleles and genotypes in patients with sarcoidosis and tuberculosis were compared with those of the control group. The association between SACE and ACE gene I/D polymorphism was also investigated in patients with sarcoidosis 

Results: The frequency of ACE gene polymorphism in patients with sarcoidosis or tuberculosis was not different from that in con­trol subjects. There was also no association between SACE and ACE gene I/D polymorphism in patients with sarcoidosis.

Conclusion:The data did not support the suggestion that the ACE gene I/D polymorphism is a marker of risk in the sarcoidosis.

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EISSN 2979-9139