Turkish Thoracic Journal
Original Article

THERAPEUTIC TREATMENT WITH MESENCHYMAL CELLS ISOLATED FROM ABDOMINAL ADIPOSE TISSUE DOES NOT PREVENT ELASTASE-INDUCED EMPHYSEMA IN THE RAT

1.

Düzce University, Faculty of Medicine, Department of Chest Diseases, Düzce, Turkey

2.

Kırıkkale University, Faculty of Medicine, Department of Chest Diseases, Kırıkkale, Turkey

3.

Bartın State Hospital, Department of Cardiovascular Surgery, Bartın, Turkey

4.

Tosya State Hospital, Department of Infectious Diseases and Clinical Microbiology, Kastamonu, Turkey

5.

Karadeniz Technical University, Faculty of Medicine, Department of Pathology, Trabzon, Turkey

6.

Yenimahalle State Hospital, Department of Biochemistry, Ankara, Turkey

Turk Thorac J 0; 5: 0-0
DOI: 10.5152/TurkThoracJ.2019.180136
Read: 129 Downloads: 11 Published: 18 July 2019

Abstract

Background: Emphysema and chronic bronchitis have different pathophysiology but are both significant components of chronic obstructive lung disease (COPD). The levels of matrix metalloproteinase (MMP-9) in bronchoalveloar lavage fluid (BALF) and in serum are associated with emphysema.Intratracheal admnistration of elastase has been used to create a rat model of emphysema. Adipose tissue-derived mesenchymal stem cells (MSC) have been postulated to prevent or reverse emphysema but this has not been examined in the rat moel of elastase-induced emphysema.

 

Methods: In this study, 6/8- week-old 31 Wistar albino rats weighing between 250- 300 g were assessed. On day 1 animals were treated intratracheally with 0.5 mls saline (Controls, n=10); 0.5 ml saline solution containing 0.1 IU porcine pancreatic elastase (PPE) per animal (Elastase group, n=12) or PPE plus MSC (Elastase-MSC group, n=9). MSCs suspended in serum were injected via the caudal vein at day 21. At least 10⁶ cell were injected. All animals were sacrificed on day 42 and the emphysema index (EI) was calculated and BALF and serum MMP-9 concentrations measured.

 

Results: PPE induced a significant degree of emphysema compared to controls as determined by the EI index (p=0.008). This was not reversed by MSC treatment and the EI remained significantly reduced compared to controls (p=0.001) and no different from Elastase-treated animals. There was no statistically significant difference between serum and BALF MMP-9 levels between the control and treatment groups.

 

Conclusions: Our findings suggest that therapeutic treatment with adipose tissuederived MSC in rats has no effect on emphysema or on MMP9 expression, a marker of emphysema.

 

Cite this article as: Yıldız Gülhan P, Ekici MS, Niyaz M, et al. THERAPEUTIC TREATMENT WITH MESENCHYMAL CELLS ISOLATED FROM ABDOMINAL ADIPOSE TISSUE DOES NOT PREVENT ELASTASE-INDUCED EMPHYSEMA IN THE RAT. Turk Thorac J 2019; DOI: 10.5152/TurkThoracJ.2019.180136

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