Thoracic Research and Practice
Clinical Study

Transforming Growth Factor-pi Levels in Induced Sputum Samples of Asthmatic Subjects

1.

Marmara University School of Medicine, Department of Pulmonary Medicine

2.

Marmara University School of Medicine, Department of Immunology Medicine

Thorac Res Pract 2000; 1: Turkish Respiratory Journal 46-50
Read: 470 Downloads: 286 Published: 06 October 2021

The histopathology of bronchial asthma is associated with structural changes within the airways. Transforming growth factor beta TGF-P; is a potent profibrotic cytokine which may contribute to the airway remodelling. To assess the clinical importance of TGF-P; in asthma we have measured basal TGF- Pj concentrations in induced sputum and serum samples of asthmatic and healthy subjects using ELISA (PROMEGA, USA). 15 asthmatic patients (3 males, 12 females, mean age 37±13 yrs) and 5 controls were included. The mean TGF-p; was 3.09±0.21 ng/ml in sputum samples of asthmatics and 2.89±0.09 ng/ml in those of controls (p>0.05); 3-07±0.15 ng/ml in serum of asthmatics and 2.89±0.20 ng/ml in those of controls (p>0.05). There were nosignificant correlations between sputum TGF-P! concentration and number of total nonsquamous cells, number of eosinophils, number of lymphocytes, percent of lymphocytes in sputum samples, however percent of eosinophils slightly but insignificantly correlated with sputum TGF-p; levels (p<0.09, r=0.44). There was no difference between serum TGF-P; and sputum TGF-P। levels either in asthmatics or in controls. Furthermore, serum and sputum TGF-P; levels did not correlate with asthma severity. We conclude that TGF-P; levels in serum and sputum samples have no predictive value in diagnostic use and reflection of the asthma severity.

Files
EISSN 2979-9139